Journal: Journal of Cellular and Molecular Medicine

Article Title: Pre-clinical and clinical significance of heparanase in Ewing’s sarcoma

doi: 10.1111/j.1582-4934.2010.01190.x

Figure Lengend Snippet: Antitumor activity of SST0001 against TC71 Ewing’s sarcoma

Article Snippet: The Ewing’s sarcoma cell line TC71 was maintained in Iscove’s modified Dulbecco’s Medium (Lonza, Verviers, Belgium) supplemented with 10% foetal bovine serum at 37°C in 5% CO 2 atmosphere.

Techniques: Activity Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Pre-clinical and clinical significance of heparanase in Ewing’s sarcoma

doi: 10.1111/j.1582-4934.2010.01190.x

Figure Lengend Snippet: Heparanase staining in Ewing’s sarcoma patients

Article Snippet: The Ewing’s sarcoma cell line TC71 was maintained in Iscove’s modified Dulbecco’s Medium (Lonza, Verviers, Belgium) supplemented with 10% foetal bovine serum at 37°C in 5% CO 2 atmosphere.

Techniques: Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Pre-clinical and clinical significance of heparanase in Ewing’s sarcoma

doi: 10.1111/j.1582-4934.2010.01190.x

Figure Lengend Snippet: Immunohistochemical staining of heparanase in Ewing’s sarcoma specimens. Formalin-fixed, paraffin-embedded 5 μm sections were subjected to immunostaining, applying anti-heparanase polyclonal antibody (Ab 733), as described under ‘Materials and methods’. Shown are representative photomicrographs of heparanase-positive specimens categorized as weak (+1, A, B) or strong (+2, C, D) intensity. Nuclear localization of heparanase is shown in (E). Specimens that were similarly stained with pre-immune serum, or applying the above procedure but lacking the primary antibody, yielded no detectable staining (F). Original magnification: (A, C) 100×; (B, D–F) 400×.

Article Snippet: The Ewing’s sarcoma cell line TC71 was maintained in Iscove’s modified Dulbecco’s Medium (Lonza, Verviers, Belgium) supplemented with 10% foetal bovine serum at 37°C in 5% CO 2 atmosphere.

Techniques: Immunohistochemical staining, Staining, Formalin-fixed Paraffin-Embedded, Immunostaining

Journal: PLoS ONE

Article Title: High Expression of CD109 Antigen Regulates the Phenotype of Cancer Stem-Like Cells/Cancer-Initiating Cells in the Novel Epithelioid Sarcoma Cell Line ESX and Is Related to Poor Prognosis of Soft Tissue Sarcoma

doi: 10.1371/journal.pone.0084187

Figure Lengend Snippet: A. Expression of CD109 mRNA in ES cell lines. Bars represent mean±SEM. ※※※ p <0.001, determined by the Mann-Whitney test. N.S.: not significant. B. Expression of CD109 mRNA in human sarcoma cell lines. Cell lines of epithelioid sarcoma (FU-EPS-1 and VA-ES-BJ), osteosarcoma (OS2000, KIKU, NY, U2OS, Saos-2, HuO9 and HOS), Ewing sarcoma (SKES, WES and RDES), synovial sarcoma (Fuji and YaFuSS) and malignant fibrous histiocytoma (MFH2003 and MFH2004) were used. C. Expression of CD109 mRNA in human fetal tissues (upper panel) and human adult tissues (lower panel). ESX was used as a positive control. D. Immunohistochemistry of CD109 in normal adult tissues.

Article Snippet: Human osteosarcoma cell lines (NY, U2OS and HOS), human Ewing sarcoma cell lines (SKES, WES, and RDES), the human synovial sarcoma cell line FUJI, and the human ES cell line VA-ES-BJ were purchased from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, Japan) and American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, MANN-WHITNEY, Positive Control, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Calcium-Dependent Calpain Activation-Mediated Mitochondrial Dysfunction and Oxidative Stress Are Required for Cytotoxicity of Epinecidin-1 in Human Synovial Sarcoma SW982 Cells

doi: 10.3390/ijms21062109

Figure Lengend Snippet: Cytotoxicity of epinecidin-1 (epi-1) on human synovial sarcoma cells. SW982 cells were treated with indicated doses of epi-1 for 5 h. Cytotoxicity was analyzed by the trypan blue exclusion assay ( A ) and MTS/PMS assay ( B ). Cells were treated with epi-1 (6.125 μM) at different time points (0.5, 1, 3, 5 h). Cytotoxicity was analyzed by the trypan blue exclusion assay ( C ) and MTS/PMS assay ( D ). Cells were treated with epi-1 (6.125 μM) or scrambled epi-1 (scr-epi-1) (6.125 μM) for 5 h, and cytotoxicity was determined by the trypan blue exclusion assay ( E ) and MTS/PMS assay ( F ). * p < 0.05 was considered significant.

Article Snippet: Human synovial sarcoma cell line SW982 was purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Trypan Blue Exclusion Assay